Antibacterial activity of Borago officinalis extracts on bacteria isolated from burns infected

This study provides a scientific information about the aqueous and methanol extracts of Borago officinalis based on its antimicrobial potential againts gram positive and gram-negative bacteria isolated From burns infection using the broth dilution and disc diffusion mehtod. Results of this study indicate the prescence of many phytochemicals which have antimicrobial activity against broad spectrum of bacteria. The methanol extract of B. officinalis showed highest activity than aqueous ones. The minimum inhibitory concentration (MIC)of the aqueous extract on the tested organisms was25_100mg/ml while in the methanol extract ranged between 25_50mg/ml on the tested organisms and the minimum bacterial concentration (MBC) of the aqueous extract was 25-200 mg/ml while the methanol extract ranged between 25-100 mg/ml. The highest activity of methanol extract demonstrated at 100 Co, 121 Co against S.aureus, K.spp, A.hydrophila, and S. marcescens, while there was low activity against S.dysenteria and E. coli. The activity of plant extract increased at acidic pH5-3 whearus, there are slightly increased of plant extract at alkaline pH 8. B. officinalis contained. essential element (Pb, Na, K, Ca, Fe, Zn, P, Mn, Co and Cu) at different concertation. The high preformance liquid chromatograph (HPLC) analysis of B. officinalis showed some chemical compounds that have antimicrobial activity against test isolates. The result of this study demonstrate that HPLC analysis of. B. officinalis constituent revealed that htis plant have antimicrobial activity against test orgainsm and this may be suggest the use at this extract intreatment of infections disease.