Anti-microbial activity Test against E. coli using Cow's Urine

The accelerating global concern over antibiotic resistance necessitates a focused search for effective, non- conventional antimicrobial agents. This study investigated the antibacterial potential of cow urine a substance historically revered and widely utilized in traditional medicine specifically targeting the clinically relevant opportunistic pathogen, Escherichia coli (E. coli). Utilizing the established agar well diffusion method, different concentrations of fresh cow urine were evaluated for their capacity to inhibit E.coli growth. The experimental results were significant, clearly demonstrating a prominent Zone of inhibition surrounding the wells treated with cow urine. This evidence confirms that active compounds within the urine effectively suppress the propagation of the bacterial culture. Size of the inhibition zone suggests the presence of potent antimicrobial components. These findings validate traditional usage and establish cow urine as a promising, accessible source for developing novel therapeutic agents. Future research is essential to isolate Escherichiae and characterize the active constituents and fully elucidate their specific mechanism of action against E. coli cell structures.